This study was undertaken to provide an analysis of the life-cycle emissions and life-cycle energy balance of the production of ethanol from several agricultural lignocellulosic feedstocks and its subsequent use as a motor fuel in blends with gasoline. The study focuses specifically on Southern Ontario, an area with a large agricultural land base, as well as one with a large demand for motor gasoline. Energy and emission analysis was conducted in this study for a base case ethanol production volume of 225 ML per year in 2000 and 2010. Further analysis was done to investigate the effects of annual ethanol production volumes of 500 ML, 750 ML and 1,000ML. The analyses were performed for four feedstocks, switchgrass, hay, corn stover and wheat straw.